Utilizing "Omic" Technologies to Identify and Prioritize Novel Sources of Resistance to the Oomycete Pathogen Phytophthora infestans in Potato Germplasm Collections.
Identifieur interne : 000135 ( Main/Exploration ); précédent : 000134; suivant : 000136Utilizing "Omic" Technologies to Identify and Prioritize Novel Sources of Resistance to the Oomycete Pathogen Phytophthora infestans in Potato Germplasm Collections.
Auteurs : Pauline S M. Van Weymers [Royaume-Uni] ; Katie Baker [Royaume-Uni] ; Xinwei Chen [Royaume-Uni] ; Brian Harrower [Royaume-Uni] ; David E L. Cooke [Royaume-Uni] ; Eleanor M. Gilroy [Royaume-Uni] ; Paul R J. Birch [Royaume-Uni] ; Gaëtan J A. Thilliez [Royaume-Uni] ; Alison K. Lees [Royaume-Uni] ; James S. Lynott [Royaume-Uni] ; Miles R. Armstrong [Royaume-Uni] ; Gaynor Mckenzie [Royaume-Uni] ; Glenn J. Bryan [Royaume-Uni] ; Ingo Hein [Royaume-Uni]Source :
- Frontiers in plant science [ 1664-462X ] ; 2016.
Abstract
The greatest threat to potato production world-wide is late blight, caused by the oomycete pathogen Phytophthora infestans. A screen of 126 wild diploid Solanum accessions from the Commonwealth Potato Collection (CPC) with P. infestans isolates belonging to the genotype 13-A2 identified resistances in the species S. bulbocastanum, S. capsicibaccatum, S. microdontum, S. mochiquense, S. okadae, S. pinnatisectum, S. polyadenium, S. tarijense, and S. verrucosum. Effector-omics, allele mining, and diagnostic RenSeq (dRenSeq) were utilized to investigate the nature of resistances in S. okadae accessions. dRenSeq in resistant S. okadae accessions 7129, 7625, 3762, and a bulk of 20 resistant progeny confirmed the presence of full-length Rpi-vnt1.1 under stringent mapping conditions and corroborated allele mining results in the accessions 7129 and 7625 as well as Avr-vnt1 recognition in transient expression assays. In contrast, susceptible S. okadae accession 3761 and a bulk of 20 susceptible progeny lacked sequence homology in the 5' end compared to the functional Rpi-vnt1.1 gene. Further evaluation of S. okadae accessions with P. infestans isolates that have a broad spectrum of virulence demonstrated that, although S. okadae accessions 7129, 7625, and 7629 contain functional Rpi-vnt1.1, they also carry a novel resistance gene. We provide evidence that existing germplasm collections are important sources of novel resistances and that "omic" technologies such as dRenSeq-based genomics and effector-omics are efficacious tools to rapidly explore the diversity within these collections.
DOI: 10.3389/fpls.2016.00672
PubMed: 27303410
PubMed Central: PMC4882398
Affiliations:
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<front><div type="abstract" xml:lang="en">The greatest threat to potato production world-wide is late blight, caused by the oomycete pathogen Phytophthora infestans. A screen of 126 wild diploid Solanum accessions from the Commonwealth Potato Collection (CPC) with P. infestans isolates belonging to the genotype 13-A2 identified resistances in the species S. bulbocastanum, S. capsicibaccatum, S. microdontum, S. mochiquense, S. okadae, S. pinnatisectum, S. polyadenium, S. tarijense, and S. verrucosum. Effector-omics, allele mining, and diagnostic RenSeq (dRenSeq) were utilized to investigate the nature of resistances in S. okadae accessions. dRenSeq in resistant S. okadae accessions 7129, 7625, 3762, and a bulk of 20 resistant progeny confirmed the presence of full-length Rpi-vnt1.1 under stringent mapping conditions and corroborated allele mining results in the accessions 7129 and 7625 as well as Avr-vnt1 recognition in transient expression assays. In contrast, susceptible S. okadae accession 3761 and a bulk of 20 susceptible progeny lacked sequence homology in the 5' end compared to the functional Rpi-vnt1.1 gene. Further evaluation of S. okadae accessions with P. infestans isolates that have a broad spectrum of virulence demonstrated that, although S. okadae accessions 7129, 7625, and 7629 contain functional Rpi-vnt1.1, they also carry a novel resistance gene. We provide evidence that existing germplasm collections are important sources of novel resistances and that "omic" technologies such as dRenSeq-based genomics and effector-omics are efficacious tools to rapidly explore the diversity within these collections. </div>
</front>
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<Abstract><AbstractText>The greatest threat to potato production world-wide is late blight, caused by the oomycete pathogen Phytophthora infestans. A screen of 126 wild diploid Solanum accessions from the Commonwealth Potato Collection (CPC) with P. infestans isolates belonging to the genotype 13-A2 identified resistances in the species S. bulbocastanum, S. capsicibaccatum, S. microdontum, S. mochiquense, S. okadae, S. pinnatisectum, S. polyadenium, S. tarijense, and S. verrucosum. Effector-omics, allele mining, and diagnostic RenSeq (dRenSeq) were utilized to investigate the nature of resistances in S. okadae accessions. dRenSeq in resistant S. okadae accessions 7129, 7625, 3762, and a bulk of 20 resistant progeny confirmed the presence of full-length Rpi-vnt1.1 under stringent mapping conditions and corroborated allele mining results in the accessions 7129 and 7625 as well as Avr-vnt1 recognition in transient expression assays. In contrast, susceptible S. okadae accession 3761 and a bulk of 20 susceptible progeny lacked sequence homology in the 5' end compared to the functional Rpi-vnt1.1 gene. Further evaluation of S. okadae accessions with P. infestans isolates that have a broad spectrum of virulence demonstrated that, although S. okadae accessions 7129, 7625, and 7629 contain functional Rpi-vnt1.1, they also carry a novel resistance gene. We provide evidence that existing germplasm collections are important sources of novel resistances and that "omic" technologies such as dRenSeq-based genomics and effector-omics are efficacious tools to rapidly explore the diversity within these collections. </AbstractText>
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<ForeName>Glenn J</ForeName>
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<ForeName>Ingo</ForeName>
<Initials>I</Initials>
<AffiliationInfo><Affiliation>Cell and Molecular Sciences, The James Hutton Institute Dundee, UK.</Affiliation>
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<Day>27</Day>
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<affiliations><list><country><li>Royaume-Uni</li>
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<tree><country name="Royaume-Uni"><noRegion><name sortKey="Van Weymers, Pauline S M" sort="Van Weymers, Pauline S M" uniqKey="Van Weymers P" first="Pauline S M" last="Van Weymers">Pauline S M. Van Weymers</name>
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<name sortKey="Chen, Xinwei" sort="Chen, Xinwei" uniqKey="Chen X" first="Xinwei" last="Chen">Xinwei Chen</name>
<name sortKey="Cooke, David E L" sort="Cooke, David E L" uniqKey="Cooke D" first="David E L" last="Cooke">David E L. Cooke</name>
<name sortKey="Gilroy, Eleanor M" sort="Gilroy, Eleanor M" uniqKey="Gilroy E" first="Eleanor M" last="Gilroy">Eleanor M. Gilroy</name>
<name sortKey="Harrower, Brian" sort="Harrower, Brian" uniqKey="Harrower B" first="Brian" last="Harrower">Brian Harrower</name>
<name sortKey="Hein, Ingo" sort="Hein, Ingo" uniqKey="Hein I" first="Ingo" last="Hein">Ingo Hein</name>
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<name sortKey="Lynott, James S" sort="Lynott, James S" uniqKey="Lynott J" first="James S" last="Lynott">James S. Lynott</name>
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<name sortKey="Thilliez, Gaetan J A" sort="Thilliez, Gaetan J A" uniqKey="Thilliez G" first="Gaëtan J A" last="Thilliez">Gaëtan J A. Thilliez</name>
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